Agarose gel analysis of 15-40-kb PCR amplimers.
نویسندگان
چکیده
Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.
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عنوان ژورنال:
- BioTechniques
دوره 21 1 شماره
صفحات -
تاریخ انتشار 1996